| Antibodies |
| Rabbit monoclonal anti-Glut1 |
Cell Signaling Technology |
Cat#12939 |
| Mouse monoclonal anti-Glut4 |
Cell Signaling Technology |
Cat# 2213 |
| Rabbit polyclonal anti- Na, K-ATPase |
Cell Signaling Technology |
Cat# 3010 |
| Rabbit polyclonal anti-tyrosine hydroxylase |
Abcam |
Cat# ab112 |
| Rabbit monoclonal anti-Akt |
Cell Signaling Technology |
Cat# 4691 |
| Rabbit monoclonal anti-phospho-Akt (Ser473) |
Cell Signaling Technology |
Cat# 4060 |
| Rabbit polyclonal anti-AMPKα |
Cell Signaling Technology |
Cat# 2532 |
| Rabbit monoclonal anti-phospho-AMPKα (Thr172) |
Cell Signaling Technology |
Cat# 2535 |
| Rabbit polyclonal anti-UCP1 |
Abcam |
Cat# ab10983 |
| Total OXPHOS Rodent WB Antibody Cocktail |
Abcam |
Cat# ab110413 |
| Rabbit polyclonal anti-PGDS |
Abcam |
Cat# ab182784 |
| Rabbit monoclonal anti-GAPDH |
Cell Signaling Technology |
Cat# 5174S |
| Mouse monoclonal anti-α-Tubulin |
Sigma-Aldrich |
Cat# T9026 |
| Chemicals, Peptides and Recombinant Proteins |
| 1,2-Dioleoyl-rac-glycerol |
Sigma-Aldrich |
Cat# D8394 |
| Oleoyl coenzyme A lithium salt |
Sigma-Aldrich |
Cat# O1012 |
| Oleoyl [14C] Coenzyme A |
American Radiolabeled Chemicals, Inc. |
Cat# ARC 0527 |
| Oleic acid [14C] |
American Radiolabeled Chemicals, Inc. |
Cat# ARC 0297 |
| Thin Layer Chromatography Plates |
Analtech |
Cat# P43911
|
| Insulin |
Lilly Corporation |
Humulin R (U 100) |
| CL 316,243 hydrate |
Sigma-Aldrich |
Cat# C5976 |
| Glycogen from bovine liver |
Sigma-Aldrich |
Cat# G0885 |
| Protease inhibitors |
Roche |
Cat# 11873580001 |
| Phospho(enol)pyruvic acid monopotassium salt |
Sigma-Aldrich |
Cat# P7127 |
| Pyruvate Kinase from rabbit muscle |
Sigma-Aldrich |
Cat# P1506 |
| β-Nicotinamide adenine dinucleotide |
Sigma-Aldrich |
Cat# N8129 |
| Critical Commercial Assays |
| Power SYBR Green PCR Master Mix |
Life Technologies |
Cat # 4368706 |
| iScript cDNA Synthesis Kit |
Biorad |
Cat# 170-8891 |
| RNeasy Mini Kit |
QIAGEN |
Cat# 74106 |
| QIAzol Lysis Reagent |
QIAGEN |
Cat # 79306 |
| QIAshredder |
QIAGEN |
Cat# 79656 |
| RNase-Free DNase Set |
QIAGEN |
Cat# 79254 |
| SuperSignal West Pico |
ThermoFisher Scientific |
Cat# 34580 |
| SuperSignal West Femto |
ThermoFisher Scientific |
Cat# 34095 |
| Glycogen Assay Kit II |
Abcam |
Cat# ab169558 |
| Infinity Triglycerides Reagent |
Thermo Fisher |
Cat # TR22421 |
| Infinity Cholesterol Reagent |
Thermo Fisher |
Cat # TR13421 |
| Free Glycerol Reagent |
Sigma-Aldrich |
Cat # F6428 |
| HR Series NEFA-HR(2) Color Reagent A |
FUJIFILM Medical Systems |
Cat # 999-34691 |
| HR Series NEFA-HR(2) Solvent A |
FUJIFILM Medical Systems |
Cat # 995-34791 |
| HR Series NEFA-HR(2) Color Reagent B |
FUJIFILM Medical Systems |
Cat # 991-34891 |
| HR Series NEFA-HR(2) Solvent B |
FUJIFILM Medical Systems |
Cat #993-35191 |
| Experimental Models: Organisms/ Strains |
| Dgat1flox/flox mice |
(Shih et al., 2009); The Jackson Laboratory |
Cat # 017322 |
| Dgat2flox/flox mice |
(Chitraju et al., 2019); The Jackson Laboratory |
Cat # 033518 |
| Ucp1-Cre mice |
(Kong et al., 2014); The Jackson Laboratory |
Cat # 024670 |
| BA-DGAT KO mice |
This paper |
N/A |
| Other |
| Chow-diet |
PicoLab® Rodent Diet 20 |
Cat # 5053 |
| Breeder’s-diet |
PicoLab® Rodent Diet 20 |
Cat # 5058 |
| High-fat-diet |
Envigo |
Cat # TD.88137 |
