Antibodies |
XBP1 |
Biolegend |
Cat # 619501 |
Caspase-3 |
Cell Signaling |
Cat # 966 |
Caspase-8 |
Cell Signaling |
Cat # 9746 |
SAPK/JNK |
Cell Signaling |
Cat # 9252 |
Phosphor-SAPK/JNK (Thr183/185) (G9) |
Cell Signaling |
Cat # 9255 |
Actin |
Cell Signaling |
Cat # 3700 |
Tubulin |
Cell Signaling |
Cat # 5346 |
SCD1 |
Cell Signaling |
Cat # 2438 |
Cytochrome b5 |
Cell Signaling |
Cat # 30311 |
Cytochrome b5 reductase |
Sigma |
SAB4500655 |
Vinculin |
Cell Signaling |
Cat # 4650 |
BiP |
Cell Signaling |
Cat # 3177 |
Calnexin |
Cell Signaling |
Cat # 2679 |
FAS |
Cell Signaling |
Cat # 3180 |
RNF213 |
Sigma |
HPA026790 |
INSIG1 |
This paper |
N/A |
Chemicals, Peptides, and Recombinant Proteins |
BODIPY (493/503) |
Thermo Fisher |
Cat # D3922 |
SCD inhibitor |
Abcam |
Cat #142089 |
DGAT1 inhibitor |
Merck & Co., Inc. |
Liu et al., 2013 |
Triacsin C |
Santa Cruz |
Cat # sc-200574A |
4u8c |
EMD Millipore |
Cat # 412512 |
GSK2606414 |
Tocris |
Cat # 5107 |
iScript cDNA Synthesis Kit |
BioRad |
Cat # 170-8891 |
Power SYBR Green PCR Master Mix |
Life Technologies |
Cat # 4368706 |
RNeasy RNA isolation kit |
Qiagen |
Cat # 79306 |
1,2-dioleoyl-sn-glycerol |
Avanti |
800811O |
1,2-dipalmitoyl-sn-glycerol |
Avanti |
800816 |
Stearoyl [9,10-3H] Coenzyme A |
American Radiolabeled Chemicals, Inc. |
ART 0390 |
Palmitic Acid [1-14C] |
American Radiolabeled Chemicals, Inc. |
ARC 0172A |
Stearoyl coenzyme A |
Sigma Aldrich |
Cat # S0802 |
NADH |
Sigma Aldrich |
Cat # N8129 |
Protease inhibitor cocktail |
Sigma-Aldrich |
118736170001 |
PhosSTOP phosphatase inhibitor |
Sigma-Aldrich |
4906837001 |
Critical Commercial Assays |
Dead Cell Apoptosis Kit |
Thermo Fisher |
V13242 |
Experimental Models: Cell Lines |
K562 |
ATCC |
CCL-243 |
SUM159 |
Laboratory of Tomas Kirchhausen |
RRID:CVCL_5423 |
HeLa |
Laboratory of Wade Harper |
N/A |
HepG2 |
ATCC |
N/A |
Oligonucleotides |
RNF213 siRNA |
Dharmacon |
D-001220-01-05 |
RISC-free (Control) siRNA |
Dharmacon |
L-023324-00-0005 |
For primers for qPCR and cloning, see Table S5
|
This paper |
N/A |
Deposited Data |
Raw and processed RNAseq data |
NCBI GEO |
GSE125178 |
Software and Algorithms |
Lipid Search |
Thermo Fisher |
Taguchi et al., 2010 |
ClueGo |
Cytoscape |
http://apps.cytoscape.org/apps/cluego |