KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| XBP1 | Biolegend | Cat # 619501 |
| Caspase-3 | Cell Signaling | Cat # 966 |
| Caspase-8 | Cell Signaling | Cat # 9746 |
| SAPK/JNK | Cell Signaling | Cat # 9252 |
| Phosphor-SAPK/JNK (Thr183/185) (G9) | Cell Signaling | Cat # 9255 |
| Actin | Cell Signaling | Cat # 3700 |
| Tubulin | Cell Signaling | Cat # 5346 |
| SCD1 | Cell Signaling | Cat # 2438 |
| Cytochrome b5 | Cell Signaling | Cat # 30311 |
| Cytochrome b5 reductase | Sigma | SAB4500655 |
| Vinculin | Cell Signaling | Cat # 4650 |
| BiP | Cell Signaling | Cat # 3177 |
| Calnexin | Cell Signaling | Cat # 2679 |
| FAS | Cell Signaling | Cat # 3180 |
| RNF213 | Sigma | HPA026790 |
| INSIG1 | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BODIPY (493/503) | Thermo Fisher | Cat # D3922 |
| SCD inhibitor | Abcam | Cat #142089 |
| DGAT1 inhibitor | Merck & Co., Inc. | Liu et al., 2013 |
| Triacsin C | Santa Cruz | Cat # sc-200574A |
| 4u8c | EMD Millipore | Cat # 412512 |
| GSK2606414 | Tocris | Cat # 5107 |
| iScript cDNA Synthesis Kit | BioRad | Cat # 170-8891 |
| Power SYBR Green PCR Master Mix | Life Technologies | Cat # 4368706 |
| RNeasy RNA isolation kit | Qiagen | Cat # 79306 |
| 1,2-dioleoyl-sn-glycerol | Avanti | 800811O |
| 1,2-dipalmitoyl-sn-glycerol | Avanti | 800816 |
| Stearoyl [9,10-3H] Coenzyme A | American Radiolabeled Chemicals, Inc. | ART 0390 |
| Palmitic Acid [1-14C] | American Radiolabeled Chemicals, Inc. | ARC 0172A |
| Stearoyl coenzyme A | Sigma Aldrich | Cat # S0802 |
| NADH | Sigma Aldrich | Cat # N8129 |
| Protease inhibitor cocktail | Sigma-Aldrich | 118736170001 |
| PhosSTOP phosphatase inhibitor | Sigma-Aldrich | 4906837001 |
| Critical Commercial Assays | ||
| Dead Cell Apoptosis Kit | Thermo Fisher | V13242 |
| Experimental Models: Cell Lines | ||
| K562 | ATCC | CCL-243 |
| SUM159 | Laboratory of Tomas Kirchhausen | RRID:CVCL_5423 |
| HeLa | Laboratory of Wade Harper | N/A |
| HepG2 | ATCC | N/A |
| Oligonucleotides | ||
| RNF213 siRNA | Dharmacon | D-001220-01-05 |
| RISC-free (Control) siRNA | Dharmacon | L-023324-00-0005 |
| For primers for qPCR and cloning, see Table S5 | This paper | N/A |
| Deposited Data | ||
| Raw and processed RNAseq data | NCBI GEO | GSE125178 |
| Software and Algorithms | ||
| Lipid Search | Thermo Fisher | Taguchi et al., 2010 |
| ClueGo | Cytoscape | http://apps.cytoscape.org/apps/cluego |