Figure 9.

NaHS induces Ca²⁺ influx by activating TRPV1 in mCRC cells. (A), NaHS (100 µM) evoked an increase in [Ca²⁺]_i in the absence (Ctrl), but not in the presence, of the specific TRPV1 antagonists: capsazepine (CPZ; 10 µM, 20 min) and SB 366791 (SB; 10 µM, 20 min) and of the less specific TRPV1 blocker ruthenium red (RuR; 10 µM, 20 min). (B), mean ± SE of the percentage of cells presenting a discernible Ca²⁺ response to NaHS under the designated treatments. (C), mean ± SE the amplitude of NaHS-evoked Ca²⁺ influx under the designated treatments. One-way ANOVA analysis followed by the post-hoc Dunnett’s test was used for Statistical comparison. In Panels B and C: *** p ≤ 0.001.