Figure 6.

Opioid preconditioning reduced CTD expression. (A) CTD mRNA expression was examined by real-time PCR. Immunohistochemistry was executed in the ischemia-reperfusion treated left kidney and areas with positive staining (B) from the sham group (C), IRI (D), morphine- (E) and fentanyl-preconditioned rats (F) were quantified. Sham group is represented by the white bars, IRI by the black bars, IRI + morphine by the dark gray bars and IRI + fentanyl by the light gray bars. The average count of positive staining areas in the sham group was set as 100%. Scale bars represent 50 µm. Values are means ± S.E.M. (n = 6). # p < 0.05 vs. sham, * p < 0.05 vs. IRI.