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. 2020 Nov 14;21(22):8599. doi: 10.3390/ijms21228599

Table 2.

The primers related to mitochondrial DNA copy number used to determine the expression of genes by qRT-PCR in this study.

Gene Forward Primer Reverse Primer
Mitochondrial DNA 5’-CCA CTG TAA AGC TAA CTT AGC ATT AAC-3’ 5’-GTG ATG AGG AAT AGT GTA AGG AGT ATG G-3’
Nuclear DNA 5’-CCA GAA AAT AAA TCA GAT GGT ATG TAA CA-3’ 5’-TGG TTT AGG GTT GCT TCC-3’
Mitochondrial DNA probe 5’-JOE-CCA ACA CCT CTT TAC AGT GAA ATG CCC CA-BHQ1-3’
Nuclear DNA probe 5’-JOE-CAG CAC TTC TTT TGA GCA CAC GGT CG-BHQ1-3’