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. 2020 Nov 17;12(11):3528. doi: 10.3390/nu12113528

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PBMCs expression pattern of specific splicing machinery components after three years of follow-up in non-T2DM and incident-T2DM subjects under two healthy dietary patterns (LF diet and Med diet). Splicing machinery components altered in non-T2DM subjects (A), incident-T2DM subjects (B) or both (C). mRNA expression levels (adjusted by a normalization factor (NF) calculated from the expression level of GAPDH and ACTB) of specific spliceosome components and splicing factors in the PBMCs from all the participants included in the study. Values represent the mean ± SEM. Asterisks indicate values that significantly differ from non-T2DM subjects (t-test: *, p < 0.05; **, p < 0.01; ***, p < 0.001).