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. 2020 Nov 13;9(11):3650. doi: 10.3390/jcm9113650

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Minimum spanning tree representations of Multi-Locus Variable number tandem-repeat Analysis (MLVA) data of the 248 C. difficile isolates (a) and the 102 (CE)032 isolates (b). The circles represent unique MLVA-types and are scaled to member count. The number between the circles represents the STRDs (summed tandem repeat differences) between MLVA-types. Grey areas represent genetically related MLVA-types (STRD ≤ 10) while MLVA-types linked by a plain line are part of the same clonal complex (STRD ≤ 2). MLVA-types are color-coded according to the RT for the (a) and according to the healthcare facility for the (b). IPP: Paris child care institute.