Table 2.
On-target coverage for the exome enrichment protocols.
| On-Target Bases (%) | SureS V6 | TS DNA Na | TS DNA Ex | Nxt DNA Ex (125 bp) | Nxt DNA Ex (350 bp) | Nxt DNA Ex (450 bp) | Ill DNA Enr |
|---|---|---|---|---|---|---|---|
| Strict target | 52.37 ± 0.55 | 16.30 ± 0.14 ** | 41.98 ± 2.58 ** | 34.65 ± 0.61 ** | 25.59 ± 0.79 ** | 22.46 ± 0.80 ** | 43.79 ± 0.99 ** |
| Padding | 63.40 ± 1.02 | 24.93 ± 0.19 ** | 51.07 ± 3.15 ** | 42.66 ± 1.05 ** | 38.00 ± 1.23 ** | 33.61 ± 1.11 ** | 58.36 ± 0.60 ** |
SureS V6: SureSelectQXT Human All Exon V6, TS DNA Na: TruSeq DNA Nano, TS DNA Ex: TruSeq DNA Exome, Nxt DNA Ex: Nextera DNA Exome, Ill DNA Enr: Illumina DNA Prep with Enrichment. bp: base pair. Numbers refer to average ± standard deviation. Statistical significance of the differences between enrichment protocols compared to SureSelectQXT V6 indicated as: ns p > 0.05; * p ≤ 0.05; and ** p ≤ 0.01.