Table 6.
Qualitative assessment of whole-exome enrichment solutions. Performance is schematically represented by a color scale, where green indicates excellent, pale green indicates very good, yellow indicates fair, and red indicates poor performance. bp: base pair.
| SureSelectQXT V6 | TruSeq DNA Nano | TruSeq DNA Exome | Nextera DNA Exome (125 bp) | Nextera DNA Exome (350 bp) | Nextera DNA Exome (450 bp) | Illumina DNA Prep with Enrichment | ||
|---|---|---|---|---|---|---|---|---|
| Library prep | ||||||||
| Oligo probes | ||||||||
| Tiling | ||||||||
| Target size (Mb) | ||||||||
| Input (ng) | ||||||||
| Fragmentation | ||||||||
| Enrichment | ||||||||
| Time (days) | ||||||||
| Hybridization time (min) | ||||||||
| Cost per sample | ||||||||
| Aligned PF bases | ||||||||
| Duplicates | ||||||||
| % on-target bases | ||||||||
| % targeted bases 1× | ||||||||
| % targeted bases 10× | ||||||||
| % targeted bases 50× | ||||||||
| % targeted shared bases | 1× | |||||||
| 10× | ||||||||
| 30× | ||||||||
| 40× | ||||||||
| 50× | ||||||||
| 100× | ||||||||
| Total variants (strict target) | ||||||||
| Total variants (padding) | ||||||||
| Total variants (in shared bases) | ||||||||
| Imputed variants (strict target) | ||||||||
| Imputed variants (padding) | ||||||||