Table A1.
Summary of sequencing data after downsampling data.
| Sequencing Data. | SureS V6 | TS DNA Na | TS DNA Ex | Nxt DNA Ex (125 bp) | Nxt DNA Ex (350 bp) | Nxt DNA Ex (450 bp) | Ill DNA Enr |
|---|---|---|---|---|---|---|---|
| Total GBases a | 4.27 ± 0.0003 | 3.42 ± 0.0001 | 3.42 ± 0.001 | 3.39 ± 0.004 | 3.42 ± 0.0002 | 3.42 ± 0.001 | 3.41 ± 0.001 |
| GBases in Q30 a | 4.01 ± 0.01 | 3.13 ± 0.01 | 3.32 ± 0.003 | 3.21 ± 0.01 | 3.13 ± 0.01 | 3.10 ± 0.02 | 3.23 ± 0.01 |
| PF Mreads a | 56.59 | 45.27 | 45.27 | 45.27 | 45.27 | 45.27 | 45.27 |
| PF Mreads aligned a | 56.50 ± 0.01 | 45.02 ± 0.02 | 45.10 ± 0.07 | 45.17 ± 0.02 | 45.14 ± 0.01 | 45.11 ± 0.02 | 45.23 ± 0.005 |
| Coverage a Strict target |
36.82 ± 27.49 | 12.10 ± 13.15 | 31.37 ± 36.83 | 25.82 ± 20.71 | 19.13 ± 16.34 | 16.74 ± 14.06 | 32.85 ± 23.49 |
| Coverage a Padding |
26.76 ± 25.84 | 9.83 ± 11.14 | 20.26 ± 30.61 | 16.87 ± 18.58 | 15.08 ± 14.14 | 13.30 ± 12.22 | 23.24 ± 21.58 |
| Mapping Q b Strict target |
56.89 ± 0.03 | 56.77 ± 0.04 | 56.51 ± 0.08 | 56.61 ± 0.03 | 56.71 ± 0.04 | 56.76 ± 0.01 | 56.68 ± 0.04 |
| Mapping Q b Padding |
56.83 ± 0.04 | 57.12 ± 0.04 | 56.73 ± 0.11 | 56.97 ± 0.04 | 57.08 ± 0.04 | 57.12 ± 0.01 | 57.03 ± 0.04 |
| GC percentage b Strict target |
54.13 ± 0.17 | 49.48 ± 0.09 | 58.67 ± 1.67 | 49.63 ± 0.24 | 50.27 ± 0.33 | 49.83 ± 1.44 | 52.49 ± 0.73 |
| GC percentage b Padding |
53.80 ± 0.25 | 47.33 ± 0.12 | 58.57 ± 1.76 | 48.93 ± 0.27 | 48.89 ± 0.39 | 48.34 ± 1.60 | 51.60 ± 0.65 |
SureS V6: SureSelectQXT Human All Exon V6, TS DNA Na: TruSeq DNA Nano, TS DNA Ex: TruSeq DNA Exome, Nxt DNA Ex: Nextera DNA Exome, Ill DNA Enr: Illumina DNA Prep with Enrichment. bp: base pair. GBases: Gigabases. Q: Phred-like quality scores. PF: passing filter. Mreads: Megareads. GC: guanine-cytosine. Mapping Q: Mapping Quality. Numbers refer to average ± standard deviation. a Metrics from Picard Tools, b Metrics from Qualimap.