Table A2.
GC bias distribution by quintiles (Q) among the exome enrichment protocols.
| Quintiles | SureS V6 | TS DNA Na | TS DNA Ex | Nxt DNA Ex (125 bp) | Nxt DNA Ex (350 bp) | Nxt DNA Ex (450 bp) | Ill DNA Enr |
|---|---|---|---|---|---|---|---|
| Q1 (0–19%) | 0.04 ± 0.01 | 0.73 ± 0.02 ** | 0.02 ± 0.004 ns | 0.12 ± 0.01 ** | 0.29 ± 0.02 ** | 0.35 ± 0.06 ** | 0.08 ± 0.02 * |
| Q2 (20–39%) | 0.26 ± 0.02 | 0.81 ± 0.01 ** | 0.06 ± 0.02 ** | 0.47 ± 0.01 ** | 0.58 ± 0.02 ** | 0.66 ± 0.08 ** | 0.31 ± 0.02 ** |
| Q3 (40–59%) | 1.22 ± 0.01 | 1.08 ± 0.01 ** | 1.04 ± 0.17 ns | 1.32 ± 0.01 ** | 1.20 ± 0.01 * | 1.17 ± 0.01 ** | 1.29 ± 0.03 ** |
| Q4 (60–89%) | 6.36 ± 0.20 | 2.19 ± 0.04 ** | 10.18 ± 1.78 ** | 3.34 ± 0.11 ** | 3.35 ± 0.12 ** | 2.92 ± 0.73 ** | 5.28 ± 0.44 ** |
| Q5 (90–100%) | 2.91 ± 0.41 | 0.43 ± 0.03 ** | 1.22 ± 0.25 ** | 0.29 ± 0.03 ** | 0.36 ± 0.03 ** | 0.34 ± 0.10 ** | 0.40 ± 0.03 ** |
SureS V6: SureSelectQXT Human All Exon V6, TS DNA Na: TruSeq DNA Nano, TS DNA Ex: TruSeq DNA Exome, Nxt DNA Ex: Nextera DNA Exome, Ill DNA Enr: Illumina DNA Prep with Enrichment. bp: base pair. Q: quintile. Numbers refer to the average ± standard deviation. Statistical significance of the differences between enrichment protocols compared to SureSelectQXT V6 indicated as: ns, p > 0.05; *, p ≤ 0.05; and **, p ≤ 0.01.