Table 1.

Table showing summary results for common carp exposed to KHV either non-permissive (12 °C) or permissive temperatures (17 and 22 °C). For each temperature treatment before and after heat stress (temperature increase to 22 °C); survival rates; seroconversion at 500 and 900 degree days; the window of KHV shedding; the gene expression patterns of lysozyme g (lysG), complement c3-h1 (c3), immunoglobulin M heavy chain secretory protein (IgMsec), immunoglobulin Z2 heavy chain (IgZ2); and pairwise correlations among them are shown. +ve: positive; -ve: negative; OD: ELISA test optical density; NA: not applicable.

		12 °C	17 °C	22 °C
Before heat stress	Survival	100%	40%	0%
	Seroconversion 500 DD	Negative	Morts: Negative Survivors: Positive, OD = 0.31 ± 0.06	Negative
	KHV shedding	From 1 to 41 days Peaks: 12, 22, 36 days	From 1 to 29 days Peaks: 5, 15, 22 days	From 1 to 12 days Peak: 5 days
	lysG	No different from controls	Peak of up-regulation	Peak of up-regulation
	c3	Down-regulation pattern	Down-regulation pattern	Down-regulation pattern
	IgMsec	Down-regulation pattern	No different from controls	Down-regulation pattern
	IgZ2	Sporadic detection	Sporadic detection	Sporadic detection
	Gene correlations	IgMsec _c3: +ve	IgMsec _c3: +ve KHV_ lysG: +ve	IgMsec _c3: +ve KHV_ IgMsec: +ve
Post heat stress (22 °C)	Survival	100%	20%	NA
	Seroconversion 900 DD	Positive, OD = 0.45 ± 0.12	Morts +ve, OD = 0.35 ± 0.04 Survivors +ve, OD = 0.51 ± 0.08	NA
	KHV shedding	From 41 to 57, Peak at 42 and 50	Negative	NA
	lysG	No different from controls	No different from controls	NA
	c3	No different from controls	No different from controls	NA
	IgMsec	Up-regulation pattern	Up-regulation pattern	NA
	IgZ2	Not detected	Sporadic detection	NA
	Gene correlations	IgMsec _c3: +ve	IgMsec _c3: +ve IgMsec_lysG: +ve IgZ2_lysG: -ve IgZ2_IgMsec: -ve	NA
	KHV carrier status	100%	100%	NA