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. 2020 Nov 16;12(11):3388. doi: 10.3390/cancers12113388

Figure 2.

Figure 2

Effect of FTY720 on autophagy in human glioma U251MG cells. (a) Cells were treated with the indicated concentrations of FTY720 for 6 h. (b) U251MG cells were pretreated with 1 mM 3-MA for 30 min, and then treated with 10 μM FTY720 for 24 h. (c,d) U251MG cells were transfected with Beclin-1 (Bec-1) siRNA (c), ATG7 siRNA, (d) and/or control siRNA (Cont siRNA). Twenty-four hours after transfection, cells were treated with 10 μM FTY720 for 24 h. (e) U251MG cells were treated with 10 μM FTY720 for 12 h, and cell morphology was detected by electron microscopy. Cell morphology was detected by interference light microscopy. The mean number of microscopic images (a–e) per sample is 10, and these images were taken randomly. Cell viability was determined by XTT assay (b). Protein expression was detected by Western blotting and cell death was analyzed by LDH assay (c,d). The values in the graphs (b–d) represent the mean ± SD of three independent experiments. * p < 0.01 compared to the control. n.s. = no significance.