Table 2.
Expression vectors commonly used at MPL. Expression hosts abbreviated as follows: E. coli (E), S. cerevisiae (S), insect (I), mammalian (M). Many vectors contain a green fluorescent protein (GFP) tag that is used to assess protein expression, localisation and monodispersity before large-scale purification.
| Vector | Selection Marker (E. coli/Host) | Tagged Terminus | Protease | Tags | Expression Host(s) | Reference |
|---|---|---|---|---|---|---|
| pWaldo GFP 8His 1 | Kan/Kan | C | TEV | GFP-8His | E | [45] |
| pDD-GFP2 1 | Amp/Uracil dropout | C | TEV | GFP-8His | S | [46] |
| pOPINEneo 3C-GFP 2 | Amp/Neo | C | 3C | GFP-8His | E, I, M | Addgene plasmid # 53534 |
| pOPINEneo TEV-GFP | Amp/Neo | C | 3C | GFP-8His | E, I, M | - |
| pOPINE-BAP 2 | Amp/- | C | - | Biotin | E, I, M | Unpublished |
| pOPINS3C 2 | Amp/- | N | 3C | SUMO | E, I, M | [47], Addgene plasmid # 41115 |
| pOPINF 2 | Amp/- | N | - | 6His | E, I, M | [41], Addgene plasmid # 26042 |
| Popinj 2 | Amp/- | N | 3C | 6His-GST | E, I, M | [41], Addgene plasmid # 26045 |
| pOPIN- HALO7 2 | Amp/- | N | 3C | 6His-HALO7 | E, I, M | As for pOPINS3C, Addgene plasmid # 41117 |
| pOPINE-3C-HALO7 2 | Amp/- | C | 3C | HALO7-6His | E, I, M | Addgene plasmid # 41126, unpublished |
| pOPINEneo-3C-2Strep 2 | Amp/Neo | C | 3C | 2StrepII-8His | E, I, M | Unpublished |
| pOPINEneo-3C-3FLAG 2 | Amp/Neo | C | 3C | 3FLAG-8His | E, I, M | Unpublished |
1 These plasmids were a gift from David Drew; 2 these plasmids were a gift from Ray Owens.