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. 2020 Nov 15;10(11):2119. doi: 10.3390/ani10112119

Table 3.

Compositions and proximate chemical analysis of experimental diets.

Treatment CON H5 H10 H15 H20 H25 H30
Diet composition (g/1000 g)
Fish meal 261 234 208 181 155 128 101
Red blood cells 100 100 100 100 100 100 100
BSFL 0 50 100 150 200 250 300
Soy protein isolate 100 100 100 100 100 100 100
Wheat gluten 150 150 150 150 150 150 150
Wheat meal 145 130 117 104 89 76 63
Maltodextrin 130 130 130 130 130 130 130
Fish oil 65 55 44 34 24 14 3
Lecithin 10 10 10 10 10 10 10
Premix 1 15 15 15 15 15 15 15
Vitamin premix 2 1 1 1 1 1 1 1
Choline chloride 2 2 2 2 2 2 2
Limestone 18 18 16 14 3 11 10
Phosphate 1-Ca 0 2 4 6 8 10 12
TiO2 3 3 3 3 3 3 3
Total 1000 1000 1000 1000 1000 1000 1000
Levels of replacements by BSFL (%)
Fish meal 0 10.3 20.3 30.7 40.6 50.9 61.3
Fish oil 0 15.4 32.3 47.7 61.3 78.5 95.4
Analysed chemical feed composition (g/1000 g)
Dry matter 938.4 937.4 934.5 934.7 934.9 936.0 935.9
Crude protein 485.5 487.7 491.4 497.9 503.5 507.4 509.7
Crude fat 99.7 102.4 101 95.5 92.1 92.3 91.0
Crude fibre 6.7 9.9 13.3 16.7 19.9 23.2 26.5
Ash 82.1 82.3 82.2 80.9 81.2 80.1 80.1
Nitrogen-free extract 264.4 255.1 246.6 243.7 238.2 233.0 228.6
Ca 13.5 14.3 14.3 14.3 14.7 14.7 15.0
P 8.1 8.1 8.1 8.1 8.1 8.1 8.1
TiO2 3.0 2.8 2.9 3.2 3.1 3.2 3.4
Gross energy (MJ/kg) 20.39 20.44 20.44 20.43 20.42 20.42 20.43
Calcium:phosphorus ratio (g:g)
Ca:P ratio 1.67:1 1.77:1 1.76:1 1.76:1 1.81:1 1.81:1 1.85:1

1 Polfamix W, BASF Polska Ltd. Kutno, Poland; containing per 1 kg: vitamin A 1000000 IU, vitamin D3 200000 IU, vitamin E 1.5 g, vitamin K 0.2 g, vitamin B1 0.05 g, vitamin B2 0.4 g, vitamin B12 0.001 g, nicotinic acid 2.5 g, D-calcium pantothenate 1.0 g, choline chloride 7.5 g, folic acid 0.1 g, methionine 150.0 g, lysine 150.0 g, Fe 2.5 g, Mn 6.5 g, Cu 0.8 g, Co 0.04 g, Zn 4.0 g, and J 0.008 g. 2 Vitazol AD3EC, BIOWET Drwalew, Poland; containing per 1 kg: vitamin A 50 000 IU, vitamin D3 5000 IU, vitamin E 30.0 mg, vitamin C 100.0 mg. CON: control feed, H5: experimental feed with 5% BSFL, H10: experimental feed with 10% BSFL, H15: experimental feed with 15% BSFL, H20: experimental feed with 20% BSFL, H25: experimental feed with 25% BSFL, H30: experimental feed with 30% BSFL.