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. 2020 Nov 13;9(11):945. doi: 10.3390/pathogens9110945

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Construction of donor vectors pFastBac-VP35, pFastBac-VP4, and pFastBac-VP35-VP4 and generation of recombinant baculoviruses. (A) PCR amplification of VP35, VP4, and VP35-VP4: lane M, DNA marker; lane 1, VP35; lane 2, VP4; lane 3, VP35-VP4. (B) Analysis of recombinant plasmid: lane M, DNA marker; lanes 1–3, double-enzyme digestion of pFastBac-VP35, pFastBac-VP4, and pFastBac-VP35-VP4 with BamHI and EcoRI. (C) Recombinant bacmid was confirmed using PCR analysis with M13. Lane M, DNA marker; lane 1, Bacmid- VP35-VP4; lane 2, Bacmid-VP4; lane 3, Bacmid-VP35; lane 4, Bacmid alone. (D) Sf-9 cells transfected with recombinant bacmid. The transfected cells typically exhibited an increase in cell diameter and nucleus size, ceased growth, lysis, and signs of clearing.