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. 2020 Nov 13;9(11):945. doi: 10.3390/pathogens9110945

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Identification of recombinant baculoviruses. The PCR products corresponding to VP35, VP4, and VP35-VP4 were amplified from the genome DNA of P1, P2, and P3 recombinant baculoviruses (AcMNPV-VP35, AcMNPV-VP4, and AcMNPV-VP35-VP4). Plasmids pFastBac-VP35, pFastBac-VP4, pFastBac-VP35- VP4, and pFastBac I were used as positive and negative controls. (A) PCR amplification of VP35 from AcMNPV-VP35: lane M, DNA marker; lanes 1–3, P1–P3 AcMNPV-VP35; lane 4, pFastBac-VP35; lane 5, pFastBac I. (B) PCR amplification of VP4 from AcMNPV-VP4: lane M, DNA marker; lanes 1–3, P1–P3 AcMNPV-VP4; lane 4, pFastBac-VP4; lane 5, pFastBac I. (C) PCR amplification of VP35-VP4 from AcMNPV-VP35-VP4: lane M, DNA marker; lanes 1–3, P1–P3 AcMNPV-VP35-VP4; lane 4, pFastBac-VP35-VP4; lane 5, pFastBac I.