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. 2020 Nov 13;9(11):945. doi: 10.3390/pathogens9110945

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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qRT-PCR analysis of the expression of immune-related genes in different tissues of the rare minnow on days 1, 3, 5, 7, 14, 21, 28, and 35 after immunization with different vaccines. (A) IL-1β, (B) TLR3, (C) Mx, (D) MyD88, (E) NF-κB, (F) TNF-α, (G) IgM. The mRNA level of each gene was normalized on the basis of β-actin gene expression. * denotes significant difference (p < 0.05), and ** denotes extremely significant difference (p < 0.01). The significant difference is compared against the group CK group. The relative mRNA expression levels of the immune-related genes were calculated using the 2^−ΔΔCt method.