Table 1.

Primer sequences and real-time PCR conditions used for gut microbiota analysis.

Bacterial Group	Primer Sequence (5′-3′)	PCR Product Size (bp)	AT (°C)
Firmicutes	ATG TGG TTT AAT TCG AAG CA	126	45
	AGC TGA CGA CAA CCA TGC AC
Bacteroidetes	CAT GTG GTT TAA TTC GAT GAT	126	45
	AGC TGA CGA CAA CCA TGC AG
Clostridium	GCA CAA GCA GTG GAG T	239	45
	CTT CCT CCG TTT TGT CAA
Bacteroides	ATA GCC TTT CGA AAG RAA GAT	495	45
	CCA GTA TCA ACT GCA ATT TTA
Universal	AAA CTC AAA KGA ATT GAC GG	180	45
	CTC ACR RCA CGA GCT GAC
Enterococcus	CCC TTA TTG TTA GTT GCC GCC ATC ATT	144	50
	ACTCGT TGT ACT TCC CT TGT
Prevotella	CAC RGT AAA CGA TGG ATG CC	513	50
	GGT CGG GTT GCA GAC C
Bifidobacterium	CGC GTC YGG TGT GAA AG	244	50
	CCC CAC ATC CAG CAT CCA
Roseburia	TAC TGC ATT GGA AAC TGT CG	230	50
	CGG CAC CGA AGA GCA AT
Lactobacillus	GAG GCA GCA GTA GGG AAT CTT C	126	55
	GGC CAG TTA CTA CCT CTA TCC TTC TTC
Akkermansia	CAG CAC GTG AAG GTG GGG AC	327	55
	CCT TGC GGT TGG CTT CAG AT

Abbreviations: AT, annealing temperature; bp, base pairs; PCR, polymerase chain reaction.