Figure 2.
Confirmation of insert in transgenic lines in A. thaliana. Three independent lines of the TsBAHD overexpression construct. Each 35S::TsBAHD line has been confirmed in severe (S), intermediate (I), and weak (W) dwarf phenotypes, represented by the three bands under each 35S::TsBAHD line in corresponding order. Expected fragment size is 683 bp. Each lane contained 5 µL of PCR product and 2 µL of loading dye. LAD was 5 µL of a 1 kb DNA ladder.