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. 2020 Nov 13;9(11):398. doi: 10.3390/biology9110398

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SF1 expression in the intestine. (a,b) Representative polyps (arrow) in the small intestine of 4-month old ApcMin/+;Sf1+/− mice as viewed under the stereomicroscope. (c) Immunohistochemistry using anti-SF1 antibody of normal mouse intestine. Arrow indicates splicing factor 1 (SF1) expression in nuclei of intestinal villi and in (d) nuclei of adenoma cells of Apc^Min/+ mice. (e) Immunoblotting using anti-SF1 antibody on cell lysate from HT-29 human colon cancer cells and lysates from Apc^Min/+ (A) and ApcMin/+;Sf1+/− (AS) mouse intestines. (Bottom) GAPDH expression in the samples.