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. 2020 Nov 13;25(22):5299. doi: 10.3390/molecules25225299

Figure 2.

Figure 2

Antitumor effect of lactate calcium salt (CaLa) on colorectal cancer (CRC) cells to determine the appropriate concentration of CaLa in combination with sorafenib. (A) Morphological changes in CRC cells were observed using an optical microscope. Scale bars: 100 μm. Enlarged pictures are provided in Supplementary Figure S3. (B) Quantitative analysis of the cell viability following treatment of CRC cells with increased concentrations of CaLa. (C) Representative cell cycle profiles of the control and 2.5 mM CaLa-treated CRC cells. All results were validated based on quintuplicate analysis. * p < 0.05 and ** p < 0.001 vs. Control. Results are mean ± SD.