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. 2020 Nov 15;9(11):2484. doi: 10.3390/cells9112484

Figure 2.

Figure 2

Proper reprogramming of ribosome biogenesis at the early stage of Yamanaka reprogramming. (A) A heat map showing that the 170 hPSC-enriched (>1.5×, q < 0.05) RB genes were upreprogrammed to the pluripotent state within 48 h. (B) A heat map showing that the 11 fibroblast-enriched RB genes were not downreprogrammed to the pluripotent state up to 72 h of OSKM induction and remained clustered with fibroblasts. (C) A heat map showing that the entire set of 298 RB genes became clustered with the hPSCs upon OSKM reprogramming. H1 and H9, human embryonic stem cell lines H1 and H9, respectively. The first number after the fibroblast or treatment designations is the time of RNA harvesting after seeding of cells or treatment time by OSKM or GFP, and the second number is the lane number of RNA-seq flow cells. hESCs are highlighted in green while OSKM samples are in red. hESC = 3; fibroblasts = 4; OSKM = 2. See also Supplementary Tables S6–S10 and Supplementary Figures S5 and S6.