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. 2020 Nov 13;9(11):805. doi: 10.3390/antibiotics9110805

Table 1.

Bacterial strains, Bacteriophages, and Plasmids used in this study.

Bacterial Strains Relevant Genotype Source, Reference
E. coli strains
E. coli B Wild-type Ian Molineux collection
E. coli B40 E. coli B supD Ian Molineux collection
E. coli B3783 E. coli B ΔtrxA<>*tetA This work; recombineering pSIM18; gene replacement hsdR<>bla
E. coli K1 Wild-type Sankar Adhya collection
E. coli K12 strains:
W3110 rph-1 inv(rrnD-rrnE) Laboratory stock strain
DY330 W3110 [λ cI857 gam+bet+exo+ Δ(cro-bioA)] {Thomason et al., 2014}
T-SACK W3110 araD<>tetA-sacB-amp fliC<>cat argG:kan {Li et al., 2013}
LT976 W3110 hsdR<>bla
λ cI857 gam+bet+exo+
Δ(cro-bioA)
Lynn Thomason collection; recombineering; DY330, gene replacement hsdR<>bla
ARP3607 W3110 hsdR<>bla
λ cI857 pL g17 Δ(cro-bioA)
This work; recombineering; gene replacement LT976, (cIII-galK)<>gp17
ARP3750 W3110 hsdR<>bla ΔtrxA<>tetA
λcI857pL g17 Δ(cro-bioA)
This work
ARP3620 LT976 ΔtrxA<>tetA This work
ARP3771 LT976 supF:Tn10 This work; transduction LT976 X (P1)
ARP3816 W3110 hsdR<>bla This work; transduction W3110 X (P1) LT976
DH5α fhuA2 lac(del)U169 phoA glnV44 Φ80’ lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 Laboratory stock strain
Klebsiella pneumoniae Kpn1760 Cured of resistance plasmid pKpQIL at 42 °C Karen Frank collection
Bacteriophages and Plasmids
T7 Wild-type Sankar Adhya collection
T7 (amber267) T7 g17Q170UAG Ian Molineux collection
K1-5 Wild-type Sankar Adhya collection
Pharr Isolated from sewage Jason Gill collection
Poteet Isolated from sewage Jason Gill collection
pSIM18 HygroR pSC101 PLgam+bet+exo+ {Thomason et al., 2014}
pSIM28 HygroR pSC101, PL-gam+ (http://redrecombineering.ncifcrf.gov/)

* “<>” represents a convention used by some bacterial geneticists to denote “[gene] replaced by [gene]” (e.g., ΔtrxA<>tetA).