Table 1.
Bacterial Strains | Relevant Genotype | Source, Reference |
---|---|---|
E. coli strains | ||
E. coli B | Wild-type | Ian Molineux collection |
E. coli B40 | E. coli B supD | Ian Molineux collection |
E. coli B3783 | E. coli B ΔtrxA<>*tetA | This work; recombineering pSIM18; gene replacement hsdR<>bla |
E. coli K1 | Wild-type | Sankar Adhya collection |
E. coli K12 strains: | ||
W3110 | rph-1 inv(rrnD-rrnE) | Laboratory stock strain |
DY330 | W3110 [λ cI857 gam+bet+exo+ Δ(cro-bioA)] | {Thomason et al., 2014} |
T-SACK | W3110 araD<>tetA-sacB-amp fliC<>cat argG:kan | {Li et al., 2013} |
LT976 | W3110 hsdR<>bla λ cI857 gam+bet+exo+ Δ(cro-bioA) |
Lynn Thomason collection; recombineering; DY330, gene replacement hsdR<>bla |
ARP3607 | W3110 hsdR<>bla λ cI857 pL g17 Δ(cro-bioA) |
This work; recombineering; gene replacement LT976, (cIII-galK)<>gp17 |
ARP3750 | W3110 hsdR<>bla ΔtrxA<>tetA λcI857pL g17 Δ(cro-bioA) |
This work |
ARP3620 | LT976 ΔtrxA<>tetA | This work |
ARP3771 | LT976 supF:Tn10 | This work; transduction LT976 X (P1) |
ARP3816 | W3110 hsdR<>bla | This work; transduction W3110 X (P1) LT976 |
DH5α | fhuA2 lac(del)U169 phoA glnV44 Φ80’ lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 | Laboratory stock strain |
Klebsiella pneumoniae Kpn1760 | Cured of resistance plasmid pKpQIL at 42 °C | Karen Frank collection |
Bacteriophages and Plasmids | ||
T7 | Wild-type | Sankar Adhya collection |
T7 (amber267) | T7 g17Q170UAG | Ian Molineux collection |
K1-5 | Wild-type | Sankar Adhya collection |
Pharr | Isolated from sewage | Jason Gill collection |
Poteet | Isolated from sewage | Jason Gill collection |
pSIM18 HygroR | pSC101 PLgam+bet+exo+ | {Thomason et al., 2014} |
pSIM28 HygroR | pSC101, PL-gam+ | (http://redrecombineering.ncifcrf.gov/) |
* “<>” represents a convention used by some bacterial geneticists to denote “[gene] replaced by [gene]” (e.g., ΔtrxA<>tetA).