Table 2.
Oligonucleotides a.
| Name | Nucleotide Sequence b | Notes |
|---|---|---|
| ARP261 | TAACGCTTCACTCGAGGCGTTTTTCGTTATGTATAAAAAGGAGCACACC/ATGGCTAACGTAATTAAAACCGTTTTGACTTACCAG | λ pL g17 forward oligo |
| ARP262 | GCTTCCCAGCCAGCGTTGCGAGTGCAGTACTCATTCGTTTTATACCTCTG/ATTACTCGTTCTCCACCATGATTGCATTAGG | λ pL g17 reverse oligo |
| ARP292 | ACCAGAACTCATGGCAAGCACGTAATGAAGCCTTA CAGTTCCGTAATGAGGCTGAGACTTTCAGAAACCAA |
T7 g17Q170UAG-CAG leading strand oligo |
| ARP293 | TTGGTTTCTGAAAGTCTCAGCCTCATTACGGAA CTGTAAGGCTTCATTACGTGCTTGCCATGAGTTCTGGT |
T7 g17Q170UAG-CAG lagging strand oligo |
| ARP301 | GGCAGTGACCCGCTTCCCGTTCGTCCGTCTGTTACTCAAACGAATCAAGGAGGTGTTCTG/ATGAGCGATAAAATTATTCACCTGACTGAC | T7 g1.7<>trxA forward |
| ARP302 | CACTCTGAGCAAGATGTGAAGTCATCAGATAGGCTGTCGGCAGGTGGGGTTGACTTGAAG/TTACGCCAGGTTAGCGTCGAGGA | T7 g1.7<>trxA reverse |
| ARP309 | ACCAACACGCCAGGCTTATTCCTGTGGAGTTATAT/TCCTAATTTTTGTTGACACTCTATC | trxA<>tetA forward |
| ARP310 | TTTTTAGCGACGGGGCACCCGAACATGAAATTCCC/ATCAAAGGGAAAACTGTCCATATGC | trxA<>tetA reverse |
| ARP321 | ACCAGAACTCATGGCAAGCACGTAATGAAGC | g17am267 70 bp dsDNA forward |
| ARP322 | CGCTTGGTTTCTGAAAGTCTCAGCCTCATTACGG | g17am267 70 bp dsDNA forward |
| ARP356 | CGAAATAATCTTCTCCCTGTAGTCTCTTAGATTTACTTTAAGGAGGTCAA/ATGAGCGATAAAATTATTCACCTGACTGAC | g17<>trxA forward |
| ARP357 | AGGTACAGTCATTGTTGTTATCTGACCCTCTACCAATGTACCAGTTATTC/TTACGCCAGGTTAGCGTCGAGGA | g17<>trxA reverse |
a Oligos were obtained desalted from Integrated DNA Technologies in Coralville, IA. b “/” indicates the border between 5′ homology to recombineering targets and 3′ homology used for primer PCR. The underlined CAG or CTG are the codon positions in the oligos ARP292 and ARP293 that correct the amber mutation in g17 used in these studies.