Fig. 1.

Effect of OA treatment on colon length, fecal parameters and Histopathology in EAE mice. a Representative image of colons. b Colon length/weight ratio (colon were excised from anus to cecum, measured, and emptied before being weighed). c Percentage of water in fecal pellets collected from mice over one hour period. d Full cecum weight/body weight ratio and weight of cecal content. e Total and relative short-chain fatty acid (SCFA) concentrations in cecal contents. f Motillin protein concentration measured in colon extracts and serum samples, from mice of the indicated groups at day 23 post-immunization. Results were expressed as the mean ± SEM, n = 5–7 per group. *p < 0.001 and **p < 0.01 vs control; and ^‡‡p < 0.01 and ^‡‡‡p < 0.05 vs untreated-EAE. C, healthy mice. C + OA, healthy mice treated with OA. EAE, induced mice. EAE + OA, induced-mice treated with OA