Figure 7.
LPA1 antagonist inhibits LPA-induced nuclear factor-κB (NF-κB) activation in LPS-primed BMDMs. Cells were primed with LPS (500 ng/mL) for 4 h, followed by an exposure to LPA (1 μM) for 1 h. NF-κB activation was determined by comparing expression levels of cytosolic and nuclear NF-κB p65 based on Western blot analysis. Representative Western blots of cytosolic and nuclear NF-κB p65 (a) and quantification of NF-κB p65 translocation into the nucleus (b,c) are shown. n = 3 per group. * p < 0.05 versus control BMDMs (Veh). # p < 0.05 versus stimulated BMDMs (LPS + LPA).