Figure 6.
Examples of local burning-induced changes in respiration (RCO2) in the second leaf of pea seedlings under control conditions (a) and after preliminary treatment by sodium orthovanadate (OV) (b) and fusicoccin (FC) (c). Respiration was measured under dark conditions. The preliminary OV (0.5 mM) and FC (1 µM) treatments of the second mature leaves in seedlings were performed by incubation of the leaf (2 h) in solutions of these chemical agents. After that, these leaves were dried by filter paper and used for respiratory measurements. OV and FC were dissolved in standard solution (1 mM KCl, 0.5 mM CaCl2, and 0.1 mM NaCl). Similar incubation in the standard solution was used as a control. The arrows mark the local burning of the first mature leaf (flame, 2–3 s).