Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 13;21(22):8551. doi: 10.3390/ijms21228551

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Reduced mucin production after tissue destruction. Exemplary presentation of alcian blue-stained colon tissue sections of (A) Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl and LysM^Cre/Cre Hif-2α^fl/fl animals and (B) Hif-1α^fl/fl × Hif-2α^fl/fl and LysM^Cre/+ Hif-1α^fl/fl × Hif-2α^fl/fl animals with and without DSS treatment and quantification of histological data (C,D). No positive alcian blue staining was observed in damaged colon areas. Statistical analysis was performed with an unpaired t-test; (A): n(Control) = 3 (LysM^Cre/Cre Hif-2α^fl/fl)/5 (Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl), n(DSS) = 8 (LysM^Cre/Cre Hif-2α^fl/fl)/12 (Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl), (B): n(Control) = 2, n(DSS) = 3. counterstaining: eosin. Magnification overview image: 100×; magnification detail image: 200×; and scale bar: 100 µm, (C,D): every indicated spot depicts the quantitative analysis of a complete cross section of one colon. **: p < 0.01.

Reduced mucin production after tissue destruction. Exemplary presentation of alcian blue-stained colon tissue sections of (A) Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl and LysM^Cre/Cre Hif-2α^fl/fl animals and (B) Hif-1α^fl/fl × Hif-2α^fl/fl and LysM^Cre/+ Hif-1α^fl/fl × Hif-2α^fl/fl animals with and without DSS treatment and quantification of histological data (C,D). No positive alcian blue staining was observed in damaged colon areas. Statistical analysis was performed with an unpaired t-test; (A): n(Control) = 3 (LysM^Cre/Cre Hif-2α^fl/fl)/5 (Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl), n(DSS) = 8 (LysM^Cre/Cre Hif-2α^fl/fl)/12 (Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl), (B): n(Control) = 2, n(DSS) = 3. counterstaining: eosin. Magnification overview image: 100×; magnification detail image: 200×; and scale bar: 100 µm, (C,D): every indicated spot depicts the quantitative analysis of a complete cross section of one colon. **: p < 0.01.