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. 2020 Nov 13;21(22):8551. doi: 10.3390/ijms21228551

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Macrophages without functional HIF-2α showed a similar migration pattern compared to wildtype cells. Exemplary presentation of F4/80-stained colon tissue sections of (A) Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl and LysM^Cre/Cre Hif-2α^fl/fl animals, and (B) Hif-1α^fl/fl × Hif-2α^fl/fl and LysM^Cre/+ Hif-1α^fl/fl × Hif-2α^fl/fl animals with and without DSS treatment and an exemplary high-resolution image that shows the specificity of the staining (C). After DSS treatment, increased numbers of F4/80-positive cells were observed in the lamina propria mucosae and tela submucosa in all tissue sections. (A): n(Control) = 3 (LysM^Cre/Cre Hif-2α^fl/fl)/5 (Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl), n(DSS) = 8 (LysM^Cre/Cre Hif-2α^fl/fl)/12 (Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl); (B): n(Control) = 7, n(DSS) = 14. (A) and (B): magnification 200×, scale bar: 100 µm; (C): magnification 400×, scale bar: 50 µm.

Macrophages without functional HIF-2α showed a similar migration pattern compared to wildtype cells. Exemplary presentation of F4/80-stained colon tissue sections of (A) Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl and LysM^Cre/Cre Hif-2α^fl/fl animals, and (B) Hif-1α^fl/fl × Hif-2α^fl/fl and LysM^Cre/+ Hif-1α^fl/fl × Hif-2α^fl/fl animals with and without DSS treatment and an exemplary high-resolution image that shows the specificity of the staining (C). After DSS treatment, increased numbers of F4/80-positive cells were observed in the lamina propria mucosae and tela submucosa in all tissue sections. (A): n(Control) = 3 (LysM^Cre/Cre Hif-2α^fl/fl)/5 (Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl), n(DSS) = 8 (LysM^Cre/Cre Hif-2α^fl/fl)/12 (Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl); (B): n(Control) = 7, n(DSS) = 14. (A) and (B): magnification 200×, scale bar: 100 µm; (C): magnification 400×, scale bar: 50 µm.