Figure 7.

Myeloid knockout of HIF-2α increased the numbers of neutrophils and T-cells in inflamed colon tissue. The expression of Adgre1, Ly6g, Cd11c, Cd4, Cd8a, and Foxp3 were quantitatively determined by qPCR in the colonic tissue of DSS-treated animals. After 6 days of DSS treatment an increased expression of Ly6g, Cd4, Cd8a, and Foxp3 were observed in RNA samples from DSS-treated LysM^Cre/+ Hif-2α^fl/fl and LysM^Cre/Cre Hif-2α^fl/f animals. Statistical analysis was performed with an unpaired t-test (mean values ± SEM; (A): n = 6 (LysM^Cre/Cre Hif-2α^fl/f)/15 (Hif-2α^fl/fl, LysM^Cre/+ Hif-2α^fl/fl); (B): n = 14). All data were normalized to the untreated wildtype control. Gene expression of untreated animals did not differ markedly from the respective wildtype control and is depicted in suppl. Figure S4. *: p < 0.05.