Table 3.
Antibodies in immunohistochemistry staining.
| Antibody | Concentration | Catalog Number and Company |
|---|---|---|
| rat anti-F4/80 | 0.180556 | #MCA497B, Bio-Rad AbD Serotec GmbH, Puchheim, Germany |
| goat antirat secondary Ab | 0.388889 | #A11077, Invitrogen Thermo Fisher Scientific, Dreieich, Germany |
| goat antimyeloperoxidase | 0.180556 | # AF3667, R&D systems |
| rabbit antigoat secondary Ab | 0.388889 | # sc2774, Santa Cruz Biotechnology, Heidelberg, Germany |
| goat anti-CD3-ε | 0.215278 | #sc-1127, Santa Cruz Biotechnology, Heidelberg, Germany |
| rabbit antigoat Alexa Fluor 488 | 0.388889 | #A-11078, Thermo Fisher Scientific, Dreieich, Germany |
| Rat anti-FoxP3 | 0.111111 | # FJK-16s, eBioscience Thermo Fisher Scientific, Dreieich, Germany |
| goat antirat Alexa Fluor 568 | 0.388889 | #A-11077, Thermo Fisher Scientific, Dreieich, Germany |
| rabbit anti-HIF-1alpha (C-Term) | 1:10,000 | #CAY-10006421, Cayman Chemical Biomol GmbH, Hamburg, Germany |
| rabbit anti-HIF-2 alpha/EPAS1 | 1:10,000 | #NB100-122, Novus Biologicals, Wiesbaden, Germany |
| Polyclonal goat-antirabbit Immunoglobulin/HRP | 0.388889 | #P044801-2, DAKO Agilent Technologies, Waldbronn, Germany |