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. 2020 Nov 17;21(22):8650. doi: 10.3390/ijms21228650

Figure 4.

Figure 4

Bilirubin affects the recruitment of MDSC in the blood in the “two-hit” sepsis model. Mice were administered i.p. LPS at 5 mg/kg body weight on day 0. After 24 h, the mice were challenged with cecal ligation and puncture (CLP: 25-gauge needle and double-puncture). Next, the mice were administered bilirubin at 30 mg/kg body weight. The mice were sacrificed 24 h after CLP surgery to collect whole blood, and blood cells were stained to assess the percentages of MDSCs (CD11b+ Gr-1+). Data are shown as mean ± SD (n = 5 mice per group) and analyzed by one-way ANOVA with Tukey–Krammer multiple comparisons test using the SPSS program. * p < 0.05 versus sham. # p < 0.05 versus LPS. ‡ p < 0.05 versus “two-hit”.