Table 2.
Effect of aging time in the hydrocarbons content, expressed as quantifier area units (AU × 103/g), of donkey meat steaks aged for 14 days under vacuum conditions (n = 10 samples for each aging time).
| Ageing Time (Days) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Volatile Compound | m/z | LRI | 1 | 3 | 6 | 9 | 14 | SEM | p-Value |
| Pentane | 43 | 500 | 119.92 a | 99.34 a,b | 69.29 b,c | 38.12 c | 83.47 a,b | 6.51 | <0.001 |
| n-Hexane | 69 | 600 | 154.90 | 135.22 | 124.46 | 128.71 | 90.79 | 7.10 | 0.084 |
| Hexane, 2,2-dimethyl- | 57 | 660 | 59.22 | 51.24 | 81.76 | 72.33 | 61.21 | 4.81 | 0.270 |
| Isopropylcyclobutane | 56 | 670 | 59.76 | 40.85 | 27.63 | 33.99 | 35.41 | 3.82 | 0.125 |
| Heptane | 71 | 700 | 145.35 | 154.56 | 168.56 | 65.65 | 121.28 | 12.27 | 0.059 |
| Pentane, 2,3,4-trimethyl- | 71 | 756 | 19.48 | 23.92 | 34.00 | 38.94 | 25.68 | 3.63 | 0.492 |
| Heptane, 3,3,4-trimethyl- | 71 | 756 | 24.50 | 29.34 | 43.01 | 50.93 | 28.61 | 4.94 | 0.440 |
| Hexane, 2,2,4-trimethyl- | 57 | 804 | 42.65 | 50.42 | 81.95 | 91.10 | 45.90 | 9.55 | 0.392 |
| 2-Heptene, 3-methyl- | 70 | 817 | 54.92 | 68.88 | 102.49 | 109.74 | 54.79 | 9.14 | 0.178 |
| Octane | 85 | 800 | 938.35 | 709.11 | 778.72 | 342.71 | 569.13 | 66.36 | 0.065 |
| 2-Octene, (E)- | 112 | 833 | 39.06 a,b | 45.17 a,b | 56.26 b | 21.97 a | 29.16 a,b | 3.82 | 0.032 |
| Cyclohexane, 1,2-dimethyl- (cis/trans) | 55 | 837 | 48.70 | 51.58 | 39.03 | 28.69 | 37.46 | 3.51 | 0.222 |
| Heptane, 2,3-dimethyl- | 43 | 847 | 22.54 | 24.65 | 41.26 | 34.94 | 21.01 | 3.85 | 0.420 |
| 4-Octene, (E)- | 55 | 849 | 28.12 | 24.91 | 29.19 | 16.53 | 16.58 | 1.99 | 0.117 |
| Bicyclo[2.2.2]octane | 67 | 869 | 36.40 | 60.50 | 33.97 | 33.08 | 45.22 | 5.69 | 0.462 |
| Octane, 2-methyl- | 57 | 903 | 18.58 | 15.90 | 23.08 | 20.92 | 17.12 | 1.49 | 0.557 |
| Heptane, 3-ethyl- | 57 | 910 | 176.78 | 200.08 | 340.87 | 261.61 | 158.73 | 32.69 | 0.428 |
| Nonane, 3,7-dimethyl- | 57 | 920 | 81.04 | 98.91 | 182.68 | 140.14 | 76.77 | 16.96 | 0.254 |
| Heptane, 2,2,4-trimethyl- | 57 | 926 | 189.83 | 199.64 | 346.37 | 255.98 | 154.68 | 33.16 | 0.431 |
| Heptane, 2-methyl-3-methylene- | 57 | 935 | 25.76 | 37.88 | 50.57 | 40.64 | 31.65 | 4.71 | 0.606 |
| Octane, 3,5-dimethyl- | 57 | 940 | 183.17 | 187.43 | 306.95 | 226.50 | 150.97 | 28.76 | 0.520 |
| 3-Methyl-3-hexene | 83 | 1042 | 53.76 | 57.27 | 90.26 | 64.45 | 47.09 | 8.09 | 0.536 |
| 2-Octene, 4-ethyl-, (E)- | 69 | 982 | 152.48 | 179.33 | 263.42 | 177.96 | 160.62 | 24.90 | 0.690 |
| Heptane, 3-ethyl-5-methylene- | 70 | 989 | 256.88 | 304.39 | 469.75 | 359.75 | 281.88 | 42.93 | 0.596 |
| 3-Ethyl-3-methylheptane | 57 | 992 | 83.30 | 86.41 | 129.37 | 96.89 | 73.42 | 11.47 | 0.632 |
| Pentane, 3,3-dimethyl- | 43 | 999 | 21.65 | 25.03 | 35.32 | 28.03 | 27.20 | 2.06 | 0.360 |
| Undecane, 6,6-dimethyl- | 57 | 1010 | 113.94 | 106.05 | 168.03 | 113.08 | 93.69 | 16.06 | 0.674 |
| Nonane, 5-methylene- | 56 | 1015 | 124.18 | 125.96 | 184.13 | 138.12 | 119.34 | 16.99 | 0.780 |
| 2-Nonene, 3-methyl-, (E)- | 70 | 1026 | 272.98 | 329.03 | 454.00 | 343.21 | 304.51 | 42.60 | 0.773 |
| Heptane, 2,2,4,6,6-pentamethyl- | 57 | 1027 | 2097.53 | 2047.35 | 2887.36 | 2376.41 | 2075.11 | 243.39 | 0.814 |
| Decane | 57 | 1000 | 469.70 | 457.81 | 617.97 | 465.74 | 448.75 | 57.99 | 0.898 |
| (Z)-4-Methyl-2-hexene | 98 | 1060 | 139.74 | 137.23 | 178.97 | 150.10 | 125.24 | 15.12 | 0.861 |
| 2,2,4,4-Tetramethyloctane | 57 | 1066 | 211.32 | 184.91 | 320.03 | 236.37 | 198.07 | 28.68 | 0.609 |
| Undecane, 5,5-dimethyl- | 57 | 1084 | 207.26 | 193.58 | 278.17 | 212.43 | 187.80 | 23.16 | 0.770 |
| Dodecane, 2,6,10-trimethyl- | 57 | 1092 | 163.67 | 134.96 | 189.02 | 154.10 | 111.87 | 13.95 | 0.513 |
| Dodecane, 4-methyl- | 43 | 1105 | 113.87 | 97.93 | 144.11 | 113.03 | 89.44 | 12.08 | 0.693 |
| 2-Decene, 3-methyl-, (Z)- | 57 | 1110 | 82.78 | 79.19 | 103.54 | 75.12 | 76.97 | 9.20 | 0.889 |
| Undecane | 57 | 1100 | 1373.12 | 1104.57 | 1573.01 | 1321.27 | 1116.49 | 127.76 | 0.774 |
| 2-Undecene, 9-methyl-, (Z)- | 57 | 1137 | 302.41 | 303.61 | 428.77 | 345.53 | 301.68 | 36.10 | 0.794 |
| 2-Acetyl-2-methyltetrahydrofuran | 57 | 1147 | 35.28 | 34.23 | 44.74 | 34.45 | 38.20 | 3.39 | 0.872 |
| 4,4-Dipropylheptane | 57 | 1161 | 14.31 | 16.74 | 20.38 | 14.75 | 15.59 | 1.02 | 0.388 |
| Pentane, 3,3-diethyl- | 57 | 1166 | 112.29 | 113.67 | 86.75 | 91.55 | 75.91 | 13.17 | 0.883 |
| Dodecane, 2-methyl-6-propyl- | 57 | 1173 | 183.67 | 157.97 | 237.76 | 206.75 | 144.99 | 19.34 | 0.582 |
| 2-Undecene, 3-methyl-, (E)- | 70 | 1181 | 57.00 | 57.19 | 75.93 | 64.50 | 54.04 | 6.32 | 0.843 |
| Dodecane | 57 | 1200 | 639.62 | 545.87 | 778.83 | 693.49 | 526.25 | 60.26 | 0.677 |
| Pentadecane, 6-methyl- | 57 | 1223 | 54.10 | 51.31 | 69.59 | 60.52 | 48.79 | 5.45 | 0.784 |
| Decane, 2,3,6-trimethyl- | 57 | 1238 | 17.80 | 15.30 | 20.01 | 15.86 | 14.27 | 0.98 | 0.393 |
| Tridecane | 71 | 1300 | 157.67 | 148.36 | 187.06 | 156.25 | 121.01 | 13.75 | 0.710 |
| Tridecane, 3-methyl- | 85 | 1304 | 16.57 | 19.63 | 21.41 | 17.97 | 13.53 | 1.21 | 0.311 |
| Tetradecane | 57 | 1400 | 23.85 | 25.28 | 19.53 | 17.16 | 14.76 | 1.77 | 0.271 |
| Total Linear Hydrocarbons | 10,311.94 | 9379.77 | 12,983.62 | 10,142.36 | 8707.29 | 1004.34 | 0.746 | ||
Different letters in the same line show statistical differences (a,b,c: p < 0.01). SEM: standard error of mean; m/z: Quantifier ion; LRI: Lineal Retention Index calculated for DB-624 capillary column (J&W scientific: 30 m × 0.25 mm id, 1.4 µm film thickness) installed on a gas chromatograph equipped with a mass selective detector. LRI: linear retention index in agreement with literature for the same chromatographic column [25,34,43].