Table 1.
Composition (g/kg as fed) of nitrogen-free diet used to estimate basal ileal endogenous amino acid losses and the assay diets for the determination of apparent ileal amino acid digestibility of the pea variants.
| Ingredient | N-Free Diet | Pea-Based Assay Diets 1 | |
|---|---|---|---|
| WF | CF | ||
| Test pea | - | 810 | 710 |
| Maize starch | 200 | 50 | 100 |
| Dextrose | 647 | 50 | 100 |
| Cellulose | 50 | - | - |
| Refined rapeseed oil | 40 | 40 | 40 |
| Ca(H2PO4)2 | 19 | 19 | 19 |
| Limestone | 18 | 16 | 16 |
| NaHCO3 | 7 | 2 | 2 |
| Vitamin-trace mineral premix 2 | 5 | 5 | 5 |
| KCl | 3 | - | - |
| K2CO3 | 2 | - | - |
| NaCl | 2 | 3 | 3 |
| MgO | 2 | - | - |
| Indigestible marker (Cr2O3) | 5 | 5 | 5 |
1 The diets were offered without or with the microbial serine protease product added on top (200 mg/kg). 2 Premix (DSM Nutritional Products Ltd., Mszczonów, Poland) provided per kilogram of diet: retinol, 3600 μg; cholecalciferol, 125 μg; α-tocopherol, 45 mg; menadione, 3 mg; thiamine, 3.25 mg; riboflavin, 7.5 mg; pyridoxine, 5 mg; cobalamin, 0.03 mg; biotin 0.25 mg; folic acid, 1.5 mg; nicotinic acid, 45 mg; calcium pantothenate, 15 mg; choline chloride, 1000 mg; Fe, 67.5 mg; Zn, 75 mg; Mn, 100 mg; Cu, 17.5 mg; I, 1 mg; Se, 0.28 mg. WF = white-flowered pea cv. Tarchalska; CF = coloured-flowered pea cv. Milwa.