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. 2020 Nov 10;21(22):8448. doi: 10.3390/ijms21228448

Figure 4.

Figure 4

(A) FACS analysis of BMMNCs, Lin− Sca1+ c-Kit+ (LSK) cells, and Flk2-CD34-HSCs in the bone marrow of recipient mice after serial transplantation. The bone marrow cells were stained with the appropriate antibodies and analyzed by FACS. Statistical analysis of the FACS data. The percentages of BMMNCs, ratios of LSK cells/BMMNCs, and ratios of Flk2-CD34-HSCs/BMMNCs in the bone marrows of primary, secondary, and tertiary recipient mice, as deduced from the FACS data, are shown in the histograms (primary transplantation, n = 3; secondary transplantation, n = 4; tertiary transplantation, n = 8). * p < 0.05, ** p < 0.01 (B) A simple model showing the negative regulatory roles of EKLF in the homeostasis of Flk2-CD34-HSCs. The factor restricts the self-renewal capability of Flk2-CD34-HSCs. It also acts as a repressor to prevent superfluous Csf2rb expression and consequent over-differentiation of Flk2-CD34-HSCs. For more details, see text.