Skip to main content
. 2020 Nov 12;25(22):5279. doi: 10.3390/molecules25225279

Figure 3.

Figure 3

Effects of Pterosin B on Ang II-activated HMGB1 secretion. H9c2 cells were treated with 300 nM Ang II with or without Pterosin B. (A) Protein expression levels of AT1R and RAGE in H9c2 cells-treated with 300 nM Ang II with or without Pterosin B for 48 h. (B,C) Quantification of AT1R and RAGE expression levels shown in (A). The protein levels were normalized to GAPDH. * p < 0.05, ** p < 0.01, *** p < 0.001. n = 6. (D) H9c2 cells were treated with Ang II for 10 min and HMGB1 expression level was detected by Western blot from cell lysate and culture medium. (E) Quantification of intracellular HMGB1 protein levels and amount of secreted HMGB1 shown in (D). * p < 0.05, ** p < 0.01, *** p < 0.001. NS vs. control. n = 4. (F) H9c2 cells were treated with Ang II with or without Pterosin B for 48 h. Western blot was performed to detect protein expression levels of TLR2, TLR4, and MyD88. (G–I) Quantification of protein levels shown in (F). * p < 0.05, *** p < 0.001. TLR2 (n = 6), TLR4 (n = 6), and MyD88 (n = 3).