Figure 4.

Pterosin B attenuates PKC-ERK activation in Ang II-induced cardiomyocyte hypertrophy. (A) H9c2 cells were treated with 300 nM Ang II for 10 min with or without Pterosin B and protein levels were analyzed by western blotting. (B) The values of the relative p-PKC (n = 6) and p-ERK (n = 7) were normalized by PKC and ERK, respectively. * p < 0.05, ** p < 0.01, *** p < 0.001. (C) H9c2 cells were treated with 300 nM Ang II for 2 h in the presence and absence of Pterosin B, and protein levels of NF-κB and p-NF-κB were examined by western blotting. (D) Quantification of protein levels shown in (C). * p < 0.05. n = 3.