Figure 5.

Anti-oxidant effect of Pterosin B in Ang II-treated H9c2 cells. (A) H9c2 cells were treated with 300 nM Ang II for 2 h with or without Pterosin B. The intracellular ROS levels were detected by CM-H₂DCFDA (upper panel) and phase-contrast image was obtained to confirm cell morphology (lower panel). P50 = Pterosin B (50 μM). Scale bar = 100 µm. (B) Quantification of the fluorescence intensity of H9c2 cells in each condition. n = 6. (C) H9c2 cells were treated with 300 nM Ang II with or without Pterosin B for 2 h and the protein expression of NOX2, NOX4, and GAPDH was examined by western blotting. (D–E) Quantification of protein levels shown in (C). The levels of protein were normalized to GAPDH expression. * p < 0.05, ** p < 0.01. n = 3.