Figure 4.

Apoptosis induction in Caco-2 cells. FACS histograms (a) and quantitative analysis of early and late apoptosis (b). Cell populations were stained with Annexin V/PI (the lower left quadrant demonstrates viable cells, upper left quadrant shows early apoptotic, the upper right quadrant represents late apoptotic and the lower right quadrant shows necrotic cells populations). Cells were treated for 48 h with Dox (IC₅₀) or ME (IC₅₀) or with the combination of ME (IC₁₀) and Dox (IC₅₀). Data represent the mean ± standard deviation of the mean of 3 independent experiments; ns (not significant), **** p < 0.0001 compared to the untreated DMSO control by one-way ANOVA.