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. 2020 Nov 16;21(22):8616. doi: 10.3390/ijms21228616

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression, reconstitution, and purification of PfPV1-Strep. (A) SDS-PAGE analysis of GST-PfPV1-Strep expression in Escherichia coli and purification (1. Marker; 2. Supernatant of cell lysate; 3. Pellet of cell lysate; 4. Flow-through fraction of Strep-Tactin column; 5. Wash (with 1% 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonate (CHAPS)) fraction; 6. Wash fraction; 7. Bing buffer containing HRV3C protease; 8. Flow through after digesting glutathione S-transferase (GST)-tag; 9. Wash fraction; 10. Elution of PfPV1-Strep). (B) Native PAGE analysis of PfPV1-Strep (1. Marker; 2. PfPV1-Strep). (C) Native PAGE analysis of rPfPV1-Strep (1. Marker; 2 rPfPV1-Strep).