Table 3.
Quantification of conventional mitotic index (MI), PHH3 MI, and Ki-67 in hot spots and overall tumor section.
| Continuous Counts | Median, All Types (Range) | Anaplastic n = 16 |
Rhabdoid n = 4 |
Papillary n = 4 |
Kruskal-Wallis ꭓ2 p-Value |
|---|---|---|---|---|---|
| Mitosis overall/HPF 1 | 1.2 (0.3–2.8) | 1.4 | 0.6 | 0.7 | 0.049 * |
| Mitosis hotspot/HPF 2 | 2.8 (1.2–6.2) | 3.4 | 1.8 | 1.9 | 0.034 * |
| PHH3 overall/HPF 1 | 1.1 (0.3–3.3) | 1.3 | 0.7 | 0.8 | 0.184 |
| PHH3 hotspot/HPF 2 | 3.0 (1.0–6.3) | 3.3 | 2.3 | 2.3 | 0.263 |
| Ki-67 hot spot/100 cells 4 | 31% (8–52%) | 34% | 18% | 18% | 0.035 * |
| Overall Ki-67 Categories | 1–10% | 10–25% | 25–50% | >50% | |
| No. of samples (% of total) 3 | 9 (38%) | 7 (29%) | 6 (25%) | 2 (8%) |
1 PHH3 and mitosis overall counting was determined by counting mitoses in 10 consecutive HPF 400× magnification. 2 PHH3 and mitosis hot spot counting was the highest number of mitoses counted in a single HPF 400× magnification. 3 Ki-67 Staining estimated by evaluating overall immunoreactivity in 20–50× magnification. 4 Ki-67 staining in hot spots evaluated by counting positive cells pr. 100 cells. * p < 0.05.