Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 13;12(11):2678. doi: 10.3390/polym12112678

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Effects of crosslinking on human epidermal keratinocytes (HEK) and dermal fibroblasts (HDF). Live/dead assay indicated the EDC crosslink scaffolds were toxic towards skin cells compared to genipin (GNP) and dihydrothermal (DHT) crosslink scaffold. (A) HDF cells and (B) HEK cells seeded on GS crosslink with GNP0.5/GNP0.5EDC30 at day 1 (D1) until day 3 (D3) (100X magnification). The white line circle indicates the dead cells. Effects of crosslinking on immunogenicity. (C) Proliferation properties of peripheral blood mononuclear cells (PBMCs) in medium supplemented with GS extracts in the carboxyfluorescein succinimidyl ester (CFSE)-labeling assay. (A–D) Representative FACS histograms of immune cells cultured with supplemented medium. Proliferation response without GS extracts was used as a control. PHA served as a positive control. PHA = phytohemagglutinin. The depicted line defines the level of proliferated immune cells. Results revealed that double crosslinking resulted in significant immune reaction in vitro. * p < 0.05 tested with Student’s t-test.