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. 2020 Nov 12;13(22):5112. doi: 10.3390/ma13225112

Figure 2.

Figure 2

(a) Efficiency of MAP DNA isolation in faecal pellets using selected isolation kits: ZR Quick-DNA Fecal Soil Microbe Microprep Kit (Zymo Research, Tustin, CA, USA), QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany), QIAamp Fast DNA Stool Mini Kit (QIAGEN, Hilden, Germany), Power Fecal DNA Kit (QIAGEN, Hilden, Germany), DNeasy Power Soil Kit (QIAGEN, Hilden, Germany), NucleoSpin DNA Stool (Macherey-Nagel, Düren, Germany), Gen Elute Stool DNA Isolation Kit (Sigma-Aldrich, St. Louis, MO, USA), Innu PREP Stool DNA kit (Analytik Jena, Berlin, Germany), MagMAX™ Total Nucleic Acid Isolation Kit (Applied Biosystems by Thermo Fisher Scientific, Vilnius, Lithuania), Nuclisens Magnetic Extraction Reagents (Biomérieux, Marcy-l’Étoile, France), MagVet Mycobacterium paratuberculosis Isolation Kit (LSI, Lissieu, France), and ID Gene Mag Paratuberculosis Extraction Kit (ID vet Genetics, Grabels, France). Error bars represent the 90th percentile and standard deviation for median and mean, respectively. (b) Efficiency of MAP DNA isolation in faecal samples using selected isolation kits; for detailed isolation kit information, see (a). Error bars represent the 90th percentile and standard deviation for median and mean, respectively.