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. 2020 Nov 12;12(11):1295. doi: 10.3390/v12111295

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Tissues from pregnant mice (brain PF and spleen PF), and placentas were assayed via RT-qPCR for viral RNA. (A) ZIKV genome copies identified per μg total RNA in spleen of pregnant females. (B) ZIKV genome copies identified per μg total RNA in brain tissue of pregnant females. (C) ZIKV genome copies identified per μg total RNA in E14.5 placenta associated with embryos infected via mother at E4.5 of development. No significant difference was detected between any of the mutants and the WT virus.