Figure 1.

Determination of protein levels building the cell junctions in THMBECs. THBMECs were cultivated for 2 and 16 days. Afterwards, total protein was isolated and separated using SDS-PAGE. Expression of proteins was detected via immuno-blotting using anti-occludin antibody (a), anti-VE-cadherin antibody (b) and anti-integrin β1 antibody (c), respectively (n = 3). THBMECs were incubated with IL1β and TNFα at a concentration of 0.05 ng/mL for 24 h. Total protein was isolated and separated using SDS-PAGE. Expression of CD31 was detected by immuno-blotting using anti-CD31 antibody (d), (n = 3).