Skip to main content
. 2020 Nov 16;9(11):3672. doi: 10.3390/jcm9113672

Figure 3.

Figure 3

Increased microbial traversal through a BBB model upon treatment with MGO (a). THBMECs were treated with 0.15 mM MGO for 1 h. 450 µL of bacteria suspension (OD 0.5) were put into each upper chamber. Medium from the lower chamber was plated on agar plates after 6 h. Graphs show the mean + SEM of counted colonies, untreated cells serve as control, (n = 3). Microbial traversal in the presence of ascorbic acid decreased in MGO-treated cells (b). THBMECs were treated with 0.15 mM MGO for 1 h. Afterwards, ascorbic acid with a concentration of 0.1 mM was administered to the treated cells. Additionally, cells were first treated with ascorbic acid for 4 h and afterwards glycated with MGO 0.15 mM for 1 h. 450 µL of E. coli suspension (OD 0.5) was put into each upper chamber. Medium from the lower chamber was plated on agar plates after 6 h. Graphs show the mean + SEM of counted colonies, untreated cells served as control, (n = 3).