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. 2020 Nov 10;9(11):1106. doi: 10.3390/antiox9111106

Figure 2.

Figure 2

The effects of peptides (a) methionine-histidine-isoleucine-arginine amide (MHIR) and (b) histidine-isoleucine-arginine-leucine amide (HIRL) on dopachrome formation with L-DOPA and mushroom tyrosinase, (c) the mushroom tyrosinase inhibitory activities of MHIR, HIRL derivatives and kojic acid. The percent tyrosinase inhibition was determined after treating 100 μM of peptides or kojic acid with L-DOPA (2.5 mM) and mushroom tyrosinase (100 µg/mL, 428 U/mL) and incubating at 25 °C for 10 min. Each experiment was conducted in triplicate and the results were averaged. (d) Lineweaver-Burk plot of tyrosinase inhibition reaction in the presence of MHIR peptide at various concentrations.