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Magnetic-resonance approaches used to investigate the helicase DnaB and the ABC transporter BmrA in presence of ATP mimics. The employed techniques comprise 2D 13C-13C NMR spectral fingerprints (A), 13C chemical-shifts perturbations (B), appearing/disappearing resonances due to dynamic changes (C), 31P NMR experiments (D) to detect bound and unbound ligands (E) and ELDOR-detected NMR experiments (F), Details are given in the text.
