Figure 10.

The inhibition of SIRT3 led to MnSOD acetylation. Inactivation of mitochondrial SIRT3 by the CCI injury induced mitochondrial MnSOD acetylation either 14 days (A) and 21 days (B) post injury as measured by immunoprecipitation. Subcutaneous infusion of BPF (50 mg/kg), concurrently with surgery (day 0), prevented MnSOD acetylation. In these conditions, prohibitin or MnSOD expression was statistically the same among the lanes. Gels are representative of results from 6 animals. Densitometric analyses of all animals per groups are reported MnSOD and acetylated MnSOD were first normalized with prohibitin and then these values were used to obtain acetylated/total MnSOD ratio. Results are expressed as mean ± SEM for 6 rats. * p < 0.05 versus sham; ^† p < 0.05 versus vehicle + CCI.